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Purely Peptides
Research Catalog

Compound monograph

Semaglutide

Semaglutide is an acylated peptide analog of glucagon-like peptide-1. Its discovery record identifies two amino-acid substitutions and derivatization at Lys26.

Published: 2026-09-04Literature/identifier verification: 2026-08-23
NN9535NNC 0113-0217GLP-1 receptor peptide analogs

01

Identity & nomenclature

Semaglutide is an acylated peptide analog of glucagon-like peptide-1. Its discovery record identifies two amino-acid substitutions and derivatization at Lys26.

PubChem lists NN9535 and NNC 0113-0217 as development identifiers for the semaglutide molecular record.

Declared aliases and development codes: NN9535, NNC 0113-0217.

02

Molecular properties

Molecular formulaC187H291N45O59
Molecular mass4114 Da
CAS Registry Number910463-68-2
PubChem CID56843331
UNII53AXN4NNHX

03

Structural characteristics

Built on the GLP-1(7-37) scaffold, retaining its C-terminal glycine. Using native GLP-1 numbering, it carries alpha-aminoisobutyric acid at position 8 in place of alanine, arginine at position 34 in place of lysine, and acylation of the lysine 26 side chain through a gamma-glutamyl unit and two AEEA spacers to a C18 fatty diacid. The position-8 substitution sits at the dipeptidyl peptidase IV cleavage site described on the native GLP-1 entries; resistance to that cleavage is the stated design rationale, not independent causal proof for each substitution. The related GLP-1(7-37) entry identifies the native structural parent. The Aib8 and Arg34 substitutions distinguish the peptide backbone from human GLP-1, while the Lys26-linked side chain is part of the declared molecular identity.

  • Aib substitution at position 8
  • Arg substitution at position 34
  • Lys26 derivatization with a fatty-diacid-containing side chain

04

Molecular targets & mechanisms

Glucagon-like peptide-1 receptor

GLP-1R

GLP-1R is a class B G-protein-coupled receptor. The cited structural and pharmacology records characterize semaglutide and retatrutide as peptide agonists at this receptor.

05

Analytical considerations

Identity assessment must account for both the peptide sequence and the Lys26-linked side chain represented in the PubChem molecular record.

06

Verified bibliography

  1. Discovery of the Once-Weekly Glucagon-Like Peptide-1 (GLP-1) Analogue Semaglutide.

    Lau J, Bloch P, Schäffer L, Pettersson I, Spetzler J, Kofoed J, Madsen K, Knudsen LB, McGuire J, Steensgaard DB, Strauss HM, Gram DX, Knudsen SM, Nielsen FS, Thygesen P, Reedtz-Runge S, Kruse T

    Journal of medicinal chemistry · 2015-09-11 · Journal Article

    current
    PMID 26308095DOI 10.1021/acs.jmedchem.5b00726SemaglutideGLP-1R
  2. Structure and dynamics of semaglutide- and taspoglutide-bound GLP-1R-Gs complexes.

    Zhang X, Belousoff MJ, Liang YL, Danev R, Sexton PM, Wootten D

    Cell reports · 2021-07-13 · Journal Article

    current
    PMID 34260945DOI 10.1016/j.celrep.2021.109374SemaglutideGLP-1R
  3. The Discovery and Development of Liraglutide and Semaglutide.

    Knudsen LB, Lau J

    Frontiers in endocrinology · 2019-04-12 · Review

    current
    PMID 31031702PMC6474072DOI 10.3389/fendo.2019.00155SemaglutideGLP-1R
  4. Semaglutide lowers body weight in rodents via distributed neural pathways.

    Gabery S, Salinas CG, Paulsen SJ, Ahnfelt-Rønne J, Alanentalo T, Baquero AF, Buckley ST, Farkas E, Fekete C, Frederiksen KS, Helms HCC, Jeppesen JF, John LM, Pyke C, Nøhr J, Lu TT, Polex-Wolf J, Prevot V, Raun K, Simonsen L, Sun G, Szilvásy-Szabó A, Willenbrock H, Secher A, Knudsen LB, Hogendorf WFJ

    JCI insight · 2020-03-26 · Journal Article

    current
    PMID 32213703PMC7213778DOI 10.1172/jci.insight.133429SemaglutideGLP-1R
  5. Pharmacology, physiology, and mechanisms of incretin hormone action.

    Campbell JE, Drucker DJ

    Cell metabolism · 2013-05-16 · Review

    current
    PMID 23684623DOI 10.1016/j.cmet.2013.04.008TirzepatideSemaglutideRetatrutideExenatideLiraglutideGLP-1RGIPR
  6. Cryo-EM structure of the activated GLP-1 receptor in complex with a G protein.

    Zhang Y, Sun B, Feng D, Hu H, Chu M, Qu Q, Tarrasch JT, Li S, Sun Kobilka T, Kobilka BK, Skiniotis G

    Nature · 2017-05-24 · Journal Article

    current
    PMID 28538729PMC5587415DOI 10.1038/nature22394SemaglutideLiraglutideExenatideTirzepatideRetatrutideGLP-1R
  7. Structural basis for ligand recognition of incretin receptors.

    Underwood CR, Parthier C, Reedtz-Runge S

    Vitamins and hormones · 2010 · Review

    current
    PMID 21094903DOI 10.1016/B978-0-12-381517-0.00009-6SemaglutideLiraglutideExenatideTirzepatideRetatrutideGLP-1RGIPR

10

Methodology & citation verification

Reference identity is checked against official PubMed metadata. Local deterministic receipts bind each normalized title and canonical metadata record to its verification date. Scientific values remain absent when an authoritative source has not been verified.

Read the full methodology →